Biotin is a water-soluble vitamin found in all organisms that functions as a cofactor of Biotin-dependent carboxylases. It belongs to the B-Complex group of Vitamins and is an essential micronutrient for all mammals. The role of Biotin (or Vitamin-H) in Carboxylases is to act as vector for carboxyl-group transfer between donor and acceptor molecules during Carboxylation reaction (Ref.1). In M. musculus (Mus musculus), Biotin is a covalently bound as a prosthetic group in Biotin-dependent Carboxylases. It is covalently attached to Carboxylases by the action of Biotin-Protein Ligase. As a co-factor Biotin changes Apocarboxylases into active Holocarboxylases. For Biotin-Protein Ligase, Biotin addition occurs as an ATP-dependent, two-step reaction that, in the first step, involves synthesis of the intermediate, Biotinyl-5 -AMP. In the [...]