In unicellular eukaryotes like S. cerevisiae (Saccharomyces cerevisiae), Biotin or Vitamin-H, a member of B-Complex group of Vitamins acts as a cofactor for a few essential enzymes of the Carboxylase family. It is also required for the biosynthesis of fatty acids and the metabolism of amino acids and carbohydrates. Some organisms, including higher plants and most fungi and bacteria, are prototrophic for Biotin. Others, including most vertebrates and some bacteria, rely on exogenous sources (Ref.1 & 2). In mammals, Biotin is supplied by intestinal bacteria. Biotin is a colorless Vitamin and Orthorhombic when crystallized. It consists of two fused rings: an Imidazol (Ureido) and a Sulfur-containing (Tetrahydrothiophene) ring; and the latter [...]