CL (Cardiolipin/Diphosphatidylglycerol) is a unique Phospholipid carrying four acyl groups and two negative charges. Chemically it is known as 1,3-bis (1 ,2 -diacyl-3 -phosphoryl-sn-glycerol)-sn-glycerol). CL is ubiquitous in eukaryotes and is required for activity of several mitochondrial enzymes and protein import. After the conversion of PA (Phosphatidate/Phosphatidic Acid) plus CTP (Cytidine Triphosphate) to CDP-DAG (Cytidine Diphosphate-Diacylglycerol) and pyrophosphate by CDS1 (CDP-DAG Synthase), CL biosynthesis in eukaryotes occurs in a three-step process in the mitochondrial inner membrane (Ref.1). First, PGS1/PGP Synthase (Phosphatidylglycerophosphate Synthase/CDP-Diacylglycerol-Glycerol-3-Phosphate 3-Phosphatidyltransferase) catalyzes the formation of PGP (Phosphatidylglycerophosphate) from CDP-DAG. In the second step, PGP is dephosphorylated to PG (Phosphatidylglycerol) by PGP Phosphatase. Lastly, CRD1 (Cardiolipin Synthetase) catalyzes a phosphatidyl transfer from CDP-DAG to PG, an irreversible reaction that involves cleavage of [...]