C. perfringens (Clostridium perfringens) is a common Gram-positive endosporeforming, non-motile, rod-shaped, anaerobic bacillus and is known to produce a variety of toxins and enzymes that are responsible for severe myonecrotic lesions. Spores survive cooking and then germinate and multiply during storage at ambient temperature, slow cooling, or inadequate re-warming. Though its natural habitats are soil and the intestinal tracts of humans and animals, it has been isolated from virtually every environment examined for its presence. C. perfringens is a human pathogen, capable of causing illness either through wound infection or food-borne intoxication (Ref.1). Alpha-toxin is regarded as the most medically important toxin produced by C. perfringens; it is the toxin primarily responsible for the prevelent disease Gas gangrene and mild Enterotoxaemia in [...]