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Spliceosomal Splicing Cycle

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During the final step in formation of a mature, functional mRNA, the introns are removed and exons are spliced together. The discovery that introns are removed during splicing came from electron microscopy of RNA-DNA hybrids between adenovirus DNA and the mRNA encoding hexon, a major virion capsid protein. For short transcription units, RNA splicing usually follows cleavage and polyadenylation of the 3’ end of the primary transcript. But for long transcription units containing multiple exons, splicing of exons in the nascent RNA usually begins before transcription of the gene is complete (Ref.1 & 2).

The splicing snRNPs (Ribonucleoproteins) associate with the pre-mRNA and with each other in an ordered sequence to form the spliceosome. This large ribonucleoprotein complex then catalyzes [...]


1.Influence of RNA secondary structure on the pre-mRNA splicing process.
Buratti E, Baralle FE.
Mol. Cell Biol. 2004 Dec; 24(24):10505-14.
2.Splicing misplaced.
Meshorer E, Misteli T.
Cell. 2005 Aug 12; 122(3):317-8.
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