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siRNA Pathway

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Description

Small interfering RNAs (siRNAs) are 21–23nt dsRNA (double-stranded RNA) molecules that facilitate potent and sequence-specific gene suppression via the mechanism of RNAi (RNA interference). When introduced into cultured mammalian cells, siRNAs facilitate the degradation of mRNA sequences to which they are homologous, thereby silencing the encoding gene. The basic mechanism behind RNAi is the breaking of a dsRNA matching a specific gene sequence into short pieces of siRNA. These siRNAs with symmetric 2–3nt 3 overhangs and 5 -phosphate and 3 -hydroxyl groups post-transcriptionally silences a gene through mRNA inhibition or degradation. Interference of gene expression by siRNA is now recognized as a naturally occurring biological strategy for silencing alleles during development in plants, invertebrates, and vertebrates (Ref.1). It is believed that the [...]

References:

1.Killing the messenger: short RNAs that silence gene expression.
Dykxhoorn DM, Novina CD, Sharp PA
Nat Rev Mol Cell Biol. 2003 Jun; 4(6): 457-67. Review.
2.RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells.
Yu JY, DeRuiter SL, Turner DL.
Proc Natl Acad Sci U S A. 2002 Apr 30; 99(9): 6047-52. Epub 2002 Apr 23.
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