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siRNA Pathway in Nematode and Mammal

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Description

Introduction of dsRNA (double-stranded RNA), that are homologous in sequence to a gene, has proven to suppress that gene’s expression through a process known as RNAi (RNA interference). The mechanism of RNAi involves the breaking of a dsRNA matching a specific gene sequence into siRNA (Small interfering RNAs). These siRNAs are 21–23nt dsRNA duplexes with symmetric 2–3nt 3 overhangs and 5 -phosphate and 3 -hydroxyl groups trigger the degradation of mRNA that match its sequence (Ref.1). Interference of gene expression by siRNA is now recognized as a naturally occurring biological strategy for silencing alleles during development in plants, invertebrates, and vertebrates. siRNAs appears to suppress gene expression without producing a non-specific cytotoxic response. It is believed that the small size of the [...]

References:

1.RNA interference is a functional pathway with therapeutic potential in human myeloid leukemia cell lines.
Cioca DP, Aoki Y, Kiyosawa K.
Cancer Gene Ther. 2003 Feb; 10(2): 125-33.
2.Expanding small RNA interference.
Tuschl T.
Nat Biotechnol. 2002 May; 20(5): 446-8. No abstract available.
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